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The Fermentation Development Laboratory is focused on modeling, optimization, control, and metabolic flux analysis of Pichia pastoris at high cell density during the production of recombinant proteins. The Fermentation Development Laboratory (FDL) is well equipped with 18 computer-controlled fermentors: 10 5L fermentors and 8 highly automated Bioengineering 22 L NLF fermentors. All 18 fermentors are integrated into a Prima VG mass spectrometer for off-gas analysis. The BPDF uses both dedicated methanol off-gas analyzers and the mass spectrometer to monitor methanol concentration during fermentation.

Capabilities

The FDL is capable of performing all types of microbial fermentation research and development. The FDL has worked with strict anaerobes ( Methanogens and Clostridium ) to high-cell density recombinant strains such as E. coli, S. cerevisiae, and Pichia pastoris . The FDL is best recognized, as is the rest of the BPDF, for its work with recombinant protein expression from the methylotrophic yeast Pichia pastoris . Over 90% of the projects in the FDL involve Pichia pastoris .

Equipment

  • 8 Bioengineering 20L NLF22 Fermentors
  • 6 New Brunswick Scientific Bioflo III Fermentors
  • 1 ILS AFC903 Fermentor
  • Bactron IV Anaerobic / Environmental Chamber
  • Prima Thermo Onix Mass Spectrometer
  • Cary 50 UV-Vis Spectrophotometer
  • LabConCo Purifier Class II Biosafety Cabinet
  • 2 Innova 4200 Series Incubator / Shakers
  •  Precision Instruments Incubator

Fermentors at BPDF